Dates in italics are the earliest or latest known dates of office, other dates are those of election and resignation or death

• 1178-1186: Osbert;
• 1200-1201: Hugh;
• 1212-1229: Alexander (died in 1229);
• 1229-1244: William;
• 1244-1249: Eudo (he had been appointed by the Bishop of Lincoln after the priory had elected Thomas de Kedrington whom the bishop refused to allow as prior due to his palsy and lack of hearing; Eudo subsequently fled to the Cistercian order rather than face the visitation of the Bishop of Lincoln);
• 1249-1272: Walter de Caddington (former sub-prior of Dunstable Priory; he resigned due to sickness whilst the Bishop of Lincoln was visiting the priory);
• 1272-1287: Matthew de Bedford;
• 1287-1313: John de Ypres ;
• 1303-1318: John de Lacu (died in 1318);
• 1318-1326: Hugh de Beauchamp;
• 1326:Roger de Wymington;
• 1338: Robert de Luftwyk;
• 1338-1348: William de Souldrop;
• 1348-1349: Richard de Hardwick (died in 1349);
• 1349-1375: Ralph de Derby (died in 1375);
• 1375-1396: Thomas de Stratford (died in 1396);
• 1396-1397: Ralph Portreeve;
• 1397-1420: Thomas Pollard;
• 1420-1425: Thomas Bole;
• 1437: John Ampthill;
• 1444: Richard;
• 1479: John Bedford;
• 1479: Richard Derby;
• 1492-1509: Thomas Cople;
• 1509-1525: Robert Hanslope (formerly sub-prior of Saint Frideswide, Oxford);
• 1525-1531: John Biggleswade (died in 1531);
• 1531-1536: Thomas Dey (formerly at Pratis Monastery, leicesterthe last prior who subscribed to the Royal Supremacy on 8th October 1534 and surrendered the priory in 1536)